ANTISEPTIC EFFECTIVENESS OF GUAVA LEAVE EXTRACT (Psidium guajava L.), TURMERIC EXTRACT (Curcuma longa L.) AND BIOPLACENTON AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

##plugins.themes.bootstrap3.article.main##

Iis Kurniati
Nina Marliana
Linda Triana
Asep Dermawan
Deny Rudiansyah
Hafizah Ilmi Sufa

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) against various antimicrobials, especially based on the insertion of mobile genetic elements, is called staphylococcal cassette chromosome mec (SCCmec) on chromosome Staphylococcus aureus.  Antiseptic is an anti-bacterial that fights pathological flora mechanically, chemically, or a combination of both, with the aim of killing, inhibiting, or reducing the number of microorganisms such as Staphylococcus aureus. Antibiotic resistance has been one of the major public health problems humans face since the discovery of antibiotics. S. aureus has been resistant to methicillin and other -lactam antibiotics such as penicillin, cephalosporin, monobactam, and carbapenem. This group of S. aureus is known as Methicillin-Resistant Staphylococcus aureus (MRSA). Infections caused by MRSA have spread rapidly and are found in almost all countries. Guava leaves (Psidium guajava L) and turmeric (Curcuma longa L) are plants in journals showing therapeutic effects such as anti-inflammatory, antioxidant, antiviral, anti-bacterial, and antifungal activities and other activities. This research was conducted to determine whether guava leaf extract and turmeric can be used as an anti-bacterial against MRSA and the minimum guava and turmeric leaf extract concentration, effectively inhibiting MRSA.


Methods: this study used guava leaf extract 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% and 20% respectively and turmeric 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% and 10%. Bioplacenton is used as a control which is commonly used as an antiseptic. The design of this study used an experimental laboratory design. The treatment group (dilution of guava leaf extract and turmeric) is contacted against MRSA, equivalent to the standard Mc Farland 0.5. MRSA planted in the media Mueller Hinton Agar (MHA) were incubated at 37 ° C for 24 hours in the hole method.


Conclusions: the results of the observations were analyzed using the ANOVA test. The minimum concentration of guava leaf extract (Psidium Guajava L), effective against MRSA, is 18%. In comparison, turmeric (Curcuma longa L) is 10%, controlling bioplacenton inhibition of 15 mm in diameter.

References

1. NSW. 2003. What is methicillin-resistant Staphylococcus aureus (MRSA). NSW Multicultural Health Communication Serve. http://mhcs.health nsw.gov.au. Diakses tgl 19 February 2012.
2. R. K. Maheshwari, A. K. Singh, J. Gaddipati, and R. C. Srimal, “Multiple biological activities of curcumin: a short review,” Life Sciences, vol. 78, no. 18, pp. 2081–2087, 2006
3. N. Niassa and C. Sittiwet, “Antimicrobial activity of Curcuma longa aqueous extract,” Journal of Pharmacology and Toxicology, vol. 4, no. 4, pp. 173– 177, 2009.
4. Noviana H. Isolasi dan uji kepekaan isolat klinis ORSA dan nonORSA terhadap vankomisin dan antibiotik lainnya. J Mikrob Indon. 2004; 9:51-4.
5. Fey PD, Salim BS, Rupp ME, Hinrichs SH, Boxrud DJ, Davis CC, dkk. Comparative molecular analysis of community or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2003;47:196-203.
6. Sunarjati, Sudigdoadi. 2010. Analysis Tipi Staphylococcal Cassette Chromosome mec (SCCmec) Isolat Methicillin-Resistant Staphylococcus aureus (MRSA). Departemen Mikrobiologi Fakultas Kedokteran Universitas Padjadjaran. Bandung. [MKB. 2010;42(4):149–54].
7. Sulastri, N.(2017). Pengaruh Pemberian Berbagai Konsentrasi Rebusan Daun Sirih Hijau (Piper betle L.) Terhadap Diameter Zona Hambat Pertumbuhan Shigella dysenteriae [Online]. Vol 17 (17), halaman. Tersedia: http://eprints.umm.ac.id/36809/2/jiptummpp-gdl-niniksulas-50043-2- babi.pdf (21 Januari 2019).
8. Arnelia.(2004). Fito-kimia Komponen Ajaib Cegah PJK, DM dan Kanker. Tersedia: http://www.kimianet.lipi.go.id/utama.cgi/artikel [29 Januari 2019].
9. Darmansyah, I.(2001). Pengobatan Diare. [Online]. Tersedia: http://www.iwandarmansjah.web.co.id [27 Januari 2019].
10. Hieronymus, B.(1998). Tanaman Obat Keluarga. Yogyakarta: Kanisius.
11. Indriani.(2006). Aktivitas Antioksidan Ekstrak Daun Jambu Biji (Psidium Guajava L.). Jurnal Ilmu Pertanian Indonesia [Online], Vol 11 (1) 4 halaman. Tersedia: http://journal.ipb.ac.id/index.php/JIPI/article/view/13917 [29} Januari 2019].
12. Richard, F.T., Joshua, A.T., & Philips, A.J.(2013). “Advancement in medicinal Plant Research." Effect of Aqueous Extract of Leaf and Bark of Guava (Psidium guajava) on Funggi Microsporum gypseum and Trichophyton mentagrophytes and Bacteria Staphylococcus aureus and Staphylococcus epidermidis. 1, (2), 45-48.
13. Razak, F.A., & Rahim, Z.H.A.(2003). "Journal of Oral Science." The Anti-adherence Effect of Piper betle and Psidium guajava Extracts on the Adhesion of Early Settlers in Dental Plaque to Salova-coated Glass Surfaces. 45, (4), 201-206.
14. Oktiarni, E.et al.(2012). “Jurnal Universitas Bengkulu”. Pengujian Ekstrak Daun Jambu Biji (Psidium Guajava Linn.) terhadap Penyembuhan Luka Bakar pada Mencit (Mus musculus). 8(1) : 1-7.
15. R. K. Maheshwari, A. K. Singh, J. Gaddipati, and R. C. Srimal, “Multiple biological activities of curcumin: a short review,” Life Sciences, vol. 78, no. 18, pp. 2081–2087, 2006
16. H. Hayakawa, Y. Minanyia, K. Ito, Y.Yamamoto, and T. Fukuda, “Difference of curcumin content in Curcuma longa L., (Zingiberaceae) caused by Hybridization with other Curcuma species,” American Journal of Plant Sciences, vol. 2, no. 2, pp. 111–119, 2011.
17. Dwitiyanti, Dwitiyanti (2015) "Daun Jambu Biji (Psidium guajava L.) sebagai Antikanker Payudara," Pharmaceutical Sciences and Research: Vol. 2 : No. 2 , Article 3. DOI: 10.7454/psr.v2i2.3323. Available at: https://scholarhub.ui.ac.id/psr/vol2/iss2/3.
18. Sangi, M.; Runtuwene, M.R.J.; Simbala, H.E.I. dan Makang, V.M.A.(2008). “Chemistry Progres”: Analisis Fitokimia Tumbuhan Obat di Kabupaten Minahasa Utara. 1,47-53.
19. Ojewole, J.A.(2006). “Methods Find Exp Clin Pharmacol." Anti-inflammatory and Analgesic Effects of Psidium guajava Linn. (Myrtaceae) Leaf Aqueous Extract in Rats and Mice. 28, (7), 441-6.

Most read articles by the same author(s)