DIFFERENCES IN THE NUMBER OF THROMBOCYTES AND THROMBOCYTE INDEX AT VARIATIONS OF TEMPERATURE AND STORAGE TIME USING HEMATOLOGY ANALYZER

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Rini Rubiarti
Betty Nurhayati
Adang Durachim
Nina Marliana

Abstract

Background: Examination of the hematology panel (hemogram) consists of leukocytes, erythrocytes, hemoglobin, hematocrit, erythrocyte index and platelets. Platelets are the smallest elements formed in the bone marrow and play an important role in the process of blood clotting. Examination of the platelet count aims to determine the number of platelets per microliter of blood. Almost all laboratories in hospitals have used a Hematology Analyzer because the examination is more practical and accurate. The specimens used were derived from venous blood with the addition of K2EDTA anticoagulant. Storage of inspection material needs to pay attention to sample stability.Examination of the platelet count can cause changes in the platelet count, and changes in the examination of the platelet index such as the average platelet volume (MPV), platelet distribution width (PDW), and thrombocytopenia (PCT). The purpose of this study was to analyze the effect of variations in temperature and storage time on the number of platelets and the platelet index examined at various temperatures using a hematology analyzer. The study covers the field of Hematology in October-November 2021. The research is analytical in nature with a quasi-experimental design. The research subjects were 30, the sampling technique was accidental sampling with primary data. The examination method uses the impedance method with the Hematology Analyzer tool. Data were analyzed using SPSS 22 version.


 


 

References

1. Kementerian Kesehatan Republik Indonesia (2011) ‘Pedoman interpretasi data klinik’. Jakarta: Kementerian Republik Indonesia.
2. Durachim, A dan Astuti, D. (2018) Hemostatis. Jakarta Selatan: Ousat Pendidikan Sumber Daya Manusia Kesehatan.
3. Gandasoebrata (2013) Penuntun Laboratorium Klinis. Jakarta: Dian Rakyat
4. Riswanto (2013) Pemeriksaan Laboratorium Hematologi. Yogyakarta: Alfamedia dan kanal Medika.
5. Wirawan (2006) Pemeriksaan Hematologi Laboratorium Sederhana. Jakarta: Balai Penerbit Fakultas Kedokteran Indonesia.
6. Sastroasmoro, Sudigdo dan Ismael, S. (2011) ‘Dasar-Dasar Metodologi Penelitian Klinis’, in. Jakarta: CV. Sagung Seto.
7. Kaufman, R. M. (2006) ‘Platelets: testing, dosing and the storage lesion--recent advances.’, Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program, pp. 492–496. doi: 10.1182/asheducation-2006.1.492.
8. Medonic, M. B. (2016) ‘Standar Operating Procedures’, Hematology Analyzer.
9. Tormey, C. A. and Stack, G. (2014) ‘Use of a cytokine-release assay to demonstrate loss of platelet secretory capacity during blood bank processing and storage’, Archives of Pathology and Laboratory Medicine, 138(11), pp. 1481–1487. doi: 10.5858/arpa.2013-0680-OA.
10. Reddoch-Cardenas, K. M. et al. (2019) ‘Cold-stored platelets: A product with function optimized for hemorrhage control’, Transfusion and Apheresis Science, 58(1), pp. 16–22. doi: 10.1016/j.transci.2018.12.012.
11. Bynum, J. A. et al. (2016) ‘Bioenergetic profiling of platelet mitochondria during storage: 4°C storage extends platelet mitochondrial function and viability’, Transfusion, 56(March), pp. S76–S84. doi: 10.1111/trf.13337.
12. LESTARI, A. Y. U. I. (2019) ‘Perbedaan Jumlah Trombosit Pada Penyimpanan Sampel Darah Suhu Ruang dan Kulkas Selama 24 Jam’. Universitas Airlangga.
13. Gunawardena, D. et al. (2017) ‘Reliability of parameters of complete blood count with different storage conditions’, Journal of clinical laboratory analysis, 31(2), p. e22042.
14. Dewi, S. R. et al. (2015) ‘Analisis Indeks Trombosit Pada Pasien Infeksi dengue’,
15. Budak, Y. U., Polat, M. and Huysal, K. (2016) ‘The use of platelet indices, plateletcrit, mean platelet volume and platelet distribution width in emergency non-traumatic abdominal surgery: a systematic review.’, Biochemia medica, 26(2), pp. 178–193.
16. Astuti, D. and Maharani, E. A. (2020) ‘Nilai Indeks Trombosit sebagai Kontrol Kualitas Komponen Konsentrat Trombosit’, Meditory, 8(4), pp. 85–94.
17. Becchi, C. et al. (2006) ‘Mean platelet volume trend in sepsis: is it a useful parameter?’, Minerva anestesiologica, 72(9), pp. 749–756

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