The Effectiveness of Eugenia polyanta Wight on Oral Microorganism in Used Toothbrushes


Neneng Nurjanah
Eliza Herijuliant
Susi Sukmasari


Background : Oral cavity is a reservoir of microorganism as a focus infection. Some of  microorganisms are transmissible of pathological disease. Toothbrushes as a medium transfer of oral microorganism   has to keep properly to prevent cross contamination or reinfection. Therefore, decontaminated toothbrush is needed to reduce numbers of microorganism. Bay leaf (Eugenia polyantha Wight) as an Indonesian’s spices is easy to find in the market. It has chemical properties consist of tannin, flavonoid, essential oils, including citric acid and eugenol.

Objective: The purpose of this study was to determine the effectiveness of Eugenia Polyantha Wight decoction against oral microorganism collected from used toothbrushes.

Methods: 150gram Eugenia polyantha Wight were boiled in 1.5 lt. distilled water in 900C for 20 minutes. It was cooled, filtrated and diluted with distilled water to become 60%, 80% and 100% of decoction. 32 toothbrushes that used by children in primary school were soaked in 3 different concentration of Eugenia polyantha Wight (60%, 80%, 100%) and sterile water as a control. All samples and control were diluted with NaCl 0.85%, then inoculated twice on PCA medium using pour plate method. Full covered plates were incubated for 24-48 hours in 370C. Colony counter has been used to count the total number of oral microorganisms on 64 PCA discs. The data were analysed using Kruskal- Wallis and Mann-Whitney test.

Result: There were significance differences of numbers of microorganism between three concentration (p = 0.007) including sterile water. The mean of microorganism number in concentration 0%, 60%, 80% and 100% were 22.94, 35.96, 3.44 and 3.88 respectively. There were significance differences between 0% and 60% (p=0,006), 0% and 80% (p=0.009), 0% and 100%(p=0.08). However, there were no significance differences between three consecutive concentration: 60% and 80% (p= 0.123), 60% and 100% (p = 0.164). 80% and 100% (p=0.874).

Conclusion: Eugenia polyantha Wight has an effectiveness to reduce numbers of oral microorganism within three concentration compare to the control. However, the most effective concentration of decoction cannot be determined.


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