COMBINATION CREAM FORMULATION OF CITRONELLA AND KECOMBRANG FLOWER AND ANALGETIC TEST ON MICE WITH INFRA RED METHOD
Keywords:
Citronella, Kecombrang flower, Cream Preparation, Analgesic Effectiveness, Plantar TestAbstract
Traditionally, citronella leaves and Kecombrang flowers have been empirically proven to cure various diseases, and can relieve pain (analgesic), but this has not been scientifically proven. This is closely related to the content of various secondary metabolites and other chemicals in citronella and flowers. kecombrang include polyphenolic compounds and steroids/triterpenoids. This study tested the phytochemical screening and analgesic effectiveness test of the combination of ethanol extract of citronella and Kecombrang flower extract in the form of a cream preparation which was carried out topically on the feet of male mice induced by stimulation with infrared light on the Plantar test. The aim is to obtain alternative analgesic drugs from natural ingredients that are rational and economical.Citronella extract was prepared by percolation using 80% ethanol. Phytochemical screening was carried out on the ethanol extract of citronella (EESW) and Kecombrang flower extract (SBK). The combination of EESW and SBK was formulated into cream preparations with variations in concentration (5:5)%; (7.5:2.5)%; (2.5:7.5)%; (10:0)%; (0:10)%, physical tests were carried out, and analgesic effectiveness tests were carried out on male mice induced by infrared light on the Plantar test device, observed pain response resistance time after being given a cream preparation that had been formulated, and Hot in cream as a comparison, every 15 minutes for 180 minutes. The data obtained were calculated as analgesic power. And the data were analyzed statistically with ANOVA and Tukey tests.The results showed that the ethanol extract of citronella and Kecombrang flower extract contained secondary metabolites of alkaloids, flavonoids, steroids/triterpenoids, saponins, glycosides, and essential oils. The best cream preparation is the EESW:SBK (5:5)% formula because at 135 minutes, it has an analgesic effect that is not significantly different from cream (7.5:2.5)% and with Hot In Cream® preparations circulating in Indonesia. market.
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