UTILIZATION OF WILD PLANT SURUHAN EXTRACTS (Peperomia pellucida l. kunth) AGAINST THE AMOUNT OF FIBROBLASTS IN THE HEALING PROCESS OF WOUNDS IN MICE (Mus musculus)

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Dessy Abdullah
Helvina Amalia

Abstract

Background: The process of wound healing consists of three phases, namely inflammatory, proliferation and maturation. One of the plants that have the effect in accelerating the healing of wounds is suruhan (Peperomia pellucida) because the suruhan has a variety of active ingredients such as saponins, flavonoids, alkaloids, steroids and triterpenoids that are influential in the third phase of wound healing, especially the proliferative phase in stimulating the formation of fibroblasts. This study aims to prove the differences between the administration of the extract suruhan (Peperomia pellucida L. Kunth) and povidone-iodine 10% against the formation of the number fibroblasts in the wound healing process in mice (Mus musculus) days 3,5,7 and 14.


Method: This research is true experimental with a post-test control group design. The research sample was 32 mice (Mus musculus) male sex, healthy, 2-3 months old with a bodyweight of 20-40 g, which were divided into 2 groups, namely the treatment group (P) and the control group (K). Each group consists of 4 subgroups. The number of each mouse in the subgroup is 4 tails. The treatment group was the group given 10% suruhan extract topically once a day for 14 days. The control group was the group given 10% povidone-iodine once a day for 14 days. Skin tissue was taken and made preparations with hematoxylin-eosin staining to check the number of fibroblasts. An independent T-test analyzed the results of the study.


Result: The results of the independent t-test analysis showed p values ​​on day 3 (p = 0.011), day 5 (p = 0.273), day 7 (p = 0.000) and day 14 (p = 0.000).


Conclusion: In conclusion, there was a significant difference in the number of fibroblasts between the control group and the treatment group.

References

1. Nanda Y, Salim NM, Iskandar CD. Hispatologi Kulit Mencit (Mus musculus) Fase Remodeling pada Penyambuhan Luka Sayat dengan Salep Getah Jarak Pagar (Jatropha curcas Linn). J Ilm Mhs Vet. 2017;01(4):780–7.
2. Mustaqim A, Asri A, Almurdi A. Pengaruh Pemberian Gel Lidah Buaya (Aloe vera) terhadap Gambaran Histopatologi Gaster Tikus Wistar yang Diinduksi Indometasin. J Kesehat Andalas. 2018;6(3):641–5.
3. Primadina N, Basori A, Perdanakusuma DS. Proses Penyembuhan Luka Ditinjau dari Aspek Mekanisme Seluler dan Molekuler. Qanun Med - Med J Fac Med Muhammadiyah Surabaya. 2019;3(1):31–5.
4. Novyana RM, Susanti. Lidah Buaya (Aloe vera) untuk Penyembuhan Luka. J Kedokt Univ Lampung. 2016;5(4):149–53.
5. Fatimatuzzahroh F, Firani NK, Kristianto H. Efektifitas Ekstrak Bunga Cengkeh (Syzygium aromaticum) terhadap Jumlah Pembuluh Darah Kapiler pada Proses Penyembuhan Luka Insisi Fase Proliferasi. Maj Kesehat FKUB. 2016;2(2):92–8.
6. Rahmawati I. Perbedaan Efek Perawatan Luka Menggunakan Gerusan Daun Petai Cina (Leucaena glauca, benth) dan Povidon Iodine 10 % dalam Mempercepat Penyembuhan Luka Bersih pada Marmut (Cavia porcellus). J Wiyata. 2014;1(2):227–30.
7. Wulandari D, Purwaningsih D. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Suruhan (Peperomia pellucida L.Kunth) terhadap Bakteri Shigella dysentriae. J Farm Indones. 2016;13(2):171–7.
8. Majumder P, Arum Kumar K V. Establishment of Quality Parameters and Pharmacognostic Evaluation of Leaves of Peperomia pellucida (L.) HBK. Int J Pharm Pharm Sci. 2011;3(Suppl. 5):375–8.
9. Fitri N. Penggunaan Krim Ekstrak Batang dan Daun Suruhan (Peperomia pellucida L.H.B.K) dalam Proses Penyembuhan Luka Bakar pada Tikus Putih (Rattus norvegicus). Biopendix. 2015;1(1):83–91.
10. Sangadji S, Wullur AC, Bodhi W. Formulasi dan Uji Gel Ekstrak Etanol Herba Suruhan (Peperomia pellucida [L.] Kunth) terhadap Luka Bakar pada Kelinci (Oryctolagus cuniculus). NJurnal Ilm Farm. 2018;7(1):10–21.
11. Chasya SA et al. Pengaruh Pemberian Gel Doksisiklin terhadap Jumlah Fibroblas pada Proses Penyembuhan Dermatitis Paederus Akibat Racun Kumbang Tomcat (Paederus sp.) pada Mencit. J Pustaka Kesehat. 2016;4(2):200–4.
12. Islami SI, Munawir A, Astuti ISW. Efek Pemberian Membran Bakiko (Bayam- Kitosan- Kolagen) terhadap Jumlah Fibroblas pada Luka Bakar Derajat II. Hang tuah Med J [Internet]. 2018;15(2):93–111. Available from: Fakultas Kedokteran Universitas Jember
13. Wei L, Wee W, Siong J, Syamsumir D. Characterization of Anticancer, Antimicrobial, Antioxidant Properties and Chemical Compositions of Peperomia pellucida Leaf Extract. Acta Med Iran. 2011;49(10):670–4.
14. Dwita LP, Ladeska V, Ramadhani A, Augusta DR, Saufia RT. Manfaat Ekstrak Etanol Daun Remek Daging (Hemigraphis colorata W. Bull) terhadap Luka Bakar pada Tikus. J Tumbuh Obat Indones. 2020;13(1):32–41.