Ani Riyani
Eem Hayati


Background: Blood examination in the laboratory consits of 3 stages, namely pre-analytical, analytical and post-analytic. In the analytical stage, quality control is carried out using blood control materials which can be assayed and unassayed control materials. There are commercial and artificial blood control materials assayed. The stability of blood control agents depends on the use of preservative media, such as Alsever. Storage of blood in preservative media is related to changes in the metabolic, biochemical, and biomechanical structure of molecules in blood cells, such as changes in shape, deformability, osmotic fragility, ability to aggregation, and intracellular viscosity of blood cells. The most likely damage is cytoskeletal proteins in the blood cell membranes which can cause blood cells to become brittle, increase osmotic fragility and change electrolyte imbalances. A decrease in ATP in blood cells up to 80-90% during storage will cause changes in the morphology of blood cells. This study aims to analyze the appearance of hematological examination of control blood with the addition of variations in the concentration of artificial Alsever solution and determine the stability of control blood by adding variations in the concentration of artificial Alsever solution.

Methods: The research method used is a laboratory experiment.

Results: The results of the study were tested statistically with the GLM test and it can be concluded that the stability of stored blood with the addition of Alsever concentration variations showed no significant differences for the examination of erythrocyte counts, Hb levels, MCV MCH and MCHC but there were significant differences between Alsever 1, 2 and 3 on leukocyte and thrombocyte tests.

Conclusions: Control blood with the addition of artificial Alsever with variations in concentration can be used to calculate the number of erythrocytes, Hb levels, MCV MCH and MCHC, as well as the number of leukocytes and platelets with stability until day 30 Key words: Artificial Alsever, variation of concentration, control blood, stability.


1. Alemu, Yared., Alemayehu Atomsa., Zewdneh Sahlemariam. 2006. Hematology. Ethiopia: Jimma University
2. Alsever, J.B., Ainslie R. 2002. Product Information Alsever's Solution. New York: J.Med
3. Ann, Bell., Sabah Sallah. 2005. The Morphology of Human Blood Cells 7th edition. Memphis, Tennessee: University of Tennessee Health Science Center
4. Antonelou, MH., dkk. 2012. Effects of pre-storage leukoreduction on stored red blood cells signaling: a time-course evaluation from shape to proteome. Journal of Proteomics, Vol 76: 220-238
5. Asscalbiass. 2011. Buku Panduan Praktikum Biokimia Kedokteran Blok Hemato Imunologi. Purwokerto: Laboratorium Biokimia Kedokteran UNSOED
6. Asscalbiass. 2010. Buku Petunjuk Praktikum Biokimia Kedokteran Blok Digetive. Purwokerto: FK UNSOED
7. Aulia, Hanum Maulida. 2018. Uji Stabilitas PRC Dalam Larutan Alsever Buatan Ditinjau Dari Kadar Hemoglobin, Nilai Hematokrit, Jumlah dan Indeks Eritrosit. Bandung: Jurusan Analis Kesehatan Poltekkes Kemenkes RI Bandung
8. Azmi, Z. F., Nurhayati, B., Hayati, E., & Maulana, E. T. (2019). Uji Stabilitas Prc Parameter Eritrosit Dalam Larutan Alsever Buatan Dengan Penambahan Formalin. Jurnal Riset Kesehatan Poltekkes Depkes Bandung, 11(2), 280-286. Https://Doi.Org/10.34011/Juriskesbdg.V11i2.793
9. Bain, Barbara J. 2011. Blood Cell Morphology in Health and Disease. Elseiver Ltd, p: 69-99
10. Bain, BJ., Lewis SM. 2012. Basic Haematological techniques. In: Practical Haematology Eleventh Ed. London: Churcill Livingstone Elsevier, p: 41-42
11. Bakta, I Made. 2003. Hematologi Klinik Ringkas. Jakarta: Penerbit Buku Kedokteran EGC
12. Barshtein, G., dkk. 2014. Storage-induced damage to red blood cell mechanical properties can be only partially reversed by rejuvenation. Transfusion Med Hemother, Vol 41: 197-204
13. Beutler, Ernest., Wanda Kuhl., Carol. 2018. West The Osmotic Fragility of Erythrocytes After Prolonged Liquid Storage and After Reinfusion. Vol
14. Binus, University. 2015. Sign Wilcoxon Test. Accessed at pada tanggal 12 Juni 2019
15. Blasi, B., dkk. 2012. Red Blood Cell Storage and Cell Morphology. Journal of the British Blood Transfusion Society, p: 90-96
16. Bosman, GJ., dkk. 2010. Comparative proteomics of erythrocyte aging in vivo and in vitro. J Proteomics, Vol 73: 396-402
17. Bosman, GJ., dkk. 2008. Erythrocyte ageing in vivo and in vitro: structural aspects and implications for transfusion. Transfus Med, Vol 18: 335-347
18. Cancelas, JA., Dumont LJ., Maes LA. 2015. Additive solution-7 reduces the red blood cell cold storage lesion. Transfusion, Vol 55: 491-498
19. Chasis, JA., Mohandas N. 1986. Erythrocyte membrane deformability and stability: two distinct membrane properties that are independently regulated by skeletal protein associations. J Cell Biol, Vol 103: 343-350
20. Choudhury, N., Mathur Ankit. 2011. Visual Detection of Hemolysis in a Blood Bag Before Issue. Asian Journal of Transfusion Science
21. Cluitmans, JCA., dkk. 2012. Red Blood Cell Deformability During Storage: Towards Functional Proteomics and Metabolomics In The Blood Bank. Blood Transfuse, Vol 10: 12
22. Grandiosa, R., , Bouwman, M.L.,Young, T., , Mérien, M., Alfaro, A,c., (2018). Effect of antiaggregants on the in vitro viability, cell count and stability of abalone (Haliotis iris) haemocytes. Affiliations expand. PMID: 29684604. DOI: 10.1016/j.fsi.201