THE EFFECT OF STORAGE TIME AND VARIATIONS OF GLYCEROL CONCENTRATION IN FROZEN PACKED RED CELL ON ERYTHROCYTE INDICES VALUES
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Abstract
Background: Storage of Packed Red Cells depends on the anticoagulant used. Generally Packed Red Cell can last 35 days to 42 days. Storage of Packed Red Cells can be extended by a freezing process called cryopreservation. Cryopreservation is the process of preserving biological samples that are susceptible to damage and maintained at low temperatures for a long time to maintain cell structure. PRC stored using the cryopreservation method was carried out by adding one of the cryopreservation agents, namely glycerol. Glycerol used to protect red blood cells during clotting and thawing. The quality of red blood cells can be seen from several blood test parameters, one of which is the erythrocyte indices. Erythrocyte indices is a value that describes the characteristics of erythrocytes in size, content and concentration. The erythrocyte indices consists of: MCV (Mean Cospucular Volume), MCH (Mean Cospucular Haemoglobin), MCHC (Mean Cospucular Hemoglobin Concentration). The aim of this study was to determine the effect of storage time in frozen packed red cells on the erythrocyte indices value and to determine the effect of glycerol concentration in frozen packed red cells on the erythrocyte indices value.
Method: The type of research is experiment research with a quasy experiment research design. In this study, glycerol was added to Packed Red Cells with varying concentrations of 35%, 40%, and 45%, then the samples were stored frozen at -20°C for 35 days, 42 days, and 49 days. Then deglycerolization was carried out, then tested using the sample to see the erythrocyte indices. The research data were statistically analyzed using the SPSS version 22 application.
Result: The results of the statistical test of the concentration difference in the significant of MCV value, namely sig. 0.000 (p<0.05) and MCHC sig. 0.000 (p<0.05). And for MCH there is no difference, namely sig. 0.185 (p<0.05).
Conclusion: Based on the results of statistical tests, it can be concluded that there is an effect of storage time on frozen packed red cells on the erythrocyte indices value. There is an effect of 35%, 40%, and 45% glycerol concentrations on the MCV and MCHC values. However, there was no effect of MCH value on glycerol concentrations of 35%, 40%, and 45%.
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